Comparison between nucleic acid amplification tests, antigen immunofluorescence assay, and in vitro infectivity in SARS-CoV-2 diagnosisArticle Published on 2022-09-012022-11-16 Journal: Brazilian journal of microbiology : [publication o [Category] COVID19(2023년), SARS, 치료기술, [키워드] amplification analyzed Antigen antigen test Cell Concordance correlated cycle threshold Diagnosis functional greater immunofluorescence assay in vitro increased risk infect Infection initial Laboratory NAAT Nasopharyngeal swab nucleic acid amplification nucleic acid amplification test POCT point-of-care positive Rapid test response SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2. specimen Swab tested viral load quantification virus transmission [DOI] 10.1007/s42770-022-00758-6 PMC 바로가기
Expression Profile and Localization of SARS-CoV-2 Nonstructural Replicase Proteins in Infected CellsArticle Published on 2022-08-312022-11-15 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 유전자 메커니즘, 진단, 치료기술, 치료법, [키워드] acute respiratory syndrome analyzed antibody antiviral strategy caused coronavirus COVID-19 pandemic determine diagnosis of SARS-CoV-2 diagnostics different time points diffusion dsRNA expression expression profile foci function Gene Expression immunofluorescence assay indicated Infection Localization mechanism mechanisms N protein nonstructural protein nsp13 nsp14 Nsp15 NSP2 Nsp3 Nsp9 Postinfection potential molecular profile Protein protein-protein interaction Proteins provide regulated replicase replicase proteins Replication responsible SARS-CoV-2 SARS-CoV-2-infected cell subcellular distribution subcellular localization subcellular localization. suggested viral growth virus replication [DOI] 10.1128/spectrum.00744-22 PMC 바로가기
Anisodamine potently inhibits SARS-CoV-2 infection in vitro and targets its main protease아니소다민은 시험관 내에서 SARS-CoV-2 감염을 강력하게 억제하고 주요 프로테아제를 표적으로 합니다Article Published on 2022-08-062022-09-11 Journal: Biochemical and Biophysical Research Communication [Category] Coronavirus, COVID19(2023년), SARS, 치료제, [키워드] active site acute respiratory disease acute respiratory syndrome acute respiratory syndrome coronavirus Amino acid amino acid residue analyzed Anisodamine Antiviral antiviral activity antiviral agent cells Chinese co-cultured coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 Cys145 disease docking score effective drug effective drugs Gly143 H-bond HEK293 immunofluorescence assay in vitro indicated Infection inhibit M pro main protease molecular docking molecular docking studies Molecular docking study pandemic predicted protease pseudovirus entry reduced SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 main protease severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 suppressed target the SARS-CoV-2 therapeutic drug Traditional Chinese medicine treating COVID-19 Treatment treatment of COVID-19 Vero E6 VERO E6 cells viral infection Viral infection. [DOI] 10.1016/j.bbrc.2022.05.024 PMC 바로가기 [Article Type] Article
Autoantibodies in COVID-19 correlate with antiviral humoral responses and distinct immune signaturesCOVID-19의 자가항체는 항바이러스 체액 반응 및 뚜렷한 면역 신호와 상관관계가 있습니다Article Published on 2022-08-012022-09-11 Journal: Allergy [Category] COVID19(2023년), SARS, 바이오마커, 진단, 치료기술, 치료제, [키워드] acute COVID-19 acute disease acute SARS-CoV-2 infection alteration antibody Antibody titer Antibody titers antineutrophil cytoplasmic antibodies antinuclear antibodies Antiviral association autoantibodies autoantibodies. autoantibody Autoimmune autoimmune pathology B-cell breadth clinically Cohort Comorbidities comparable controls coronavirus disease Coronavirus disease 2019 correlated COVID-19 COVID-19 patient COVID-19 patients cross-reactive cytoplasmic demonstrated detect disease disease course exhibited feature healthy controls humoral immune response Humoral response humoral responses immune immune responses immune signature Immunity immunofluorescence assay implication indirect immunofluorescence individual Infection Inflammatory occur other coronavirus other coronaviruses Paired analysis Patient patients performed Prevalence proteomics SARS-CoV-2 screened serological serum severe COVID-19 severity of COVID-19 subgroup of patient trajectory vaccinated individuals VirScan [DOI] 10.1111/all.15302 PMC 바로가기 [Article Type] Article
Development of Monoclonal Antibodies to Detect for SARS-CoV-2 ProteinsSARS-CoV-2 단백질 검출을 위한 단클론항체 개발Article Published on 2022-05-302022-09-11 Journal: Journal of Molecular Biology [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, [키워드] antibodies antibody caused characterization COVID-19 pandemic develop development effort ELISA ENhance generate healthcare IFA immunofluorescence assay impacted Infection initiated interactions limit monoclonal antibody nine nsp1 Nsp12 NSP8 ORF3 pathophysiology phage display. platform Protein reagent recognize SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 protein SARS-CoV-2 proteins specific antibody specificity validation study Viral Viral protein Viral proteins western blots [DOI] 10.1016/j.jmb.2022.167583 PMC 바로가기 [Article Type] Article
Seroprevalence of anti-SARS-CoV-2 antibodies in Thai adults during the first three epidemic waves처음 3개의 전염병 파동 동안 태국 성인에서 항 SARS-CoV-2 항체의 혈청 유병률Article Published on 2022-04-272022-09-11 Journal: PLoS ONE [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] anti-SARS-CoV-2 antibodies anti-SARS-CoV-2 antibody antibody assays chemiluminescence immunoassay Community Contact tracing coronavirus coronavirus disease Coronavirus disease-19 country COVID-19 COVID-19 outbreak COVID-19 patient COVID-19 patients delta variant disease onset Disease prevalence driver Epidemic epidemic wave epidemic waves Health IgG IgG antibodies immunofluorescence assay individual individuals infection rate microneutralization N protein Neutralizing antibodies numbers of patient participant patients performed Prevalence Public public transport recruited risk SARS-CoV-2 sera seropositive Seropositivity Seroprevalence serum samples Support tested Thai people the N protein the patient Transport with COVID-19 [DOI] 10.1371/journal.pone.0263316 PMC 바로가기 [Article Type] Article
Evaluation of Antiviral Effect against SARS-CoV-2 Propagation by Crude Polysaccharides from Seaweed and Abalone Viscera In Vitro해조류 및 전복 내장의 조다당류에 의한 SARS-CoV-2 증식에 대한 시험관내 항바이러스 효과 평가Article Published on 2022-04-272022-09-11 Journal: Marine Drugs [Category] COVID19(2023년), SARS, 치료법, 치료제, [키워드] Abalone abalone viscera addition Antiviral antiviral activity Antiviral effect COVID-19 discus Effect evaluated expression level followed by Haliotis immunofluorescence assay inhibit SARS-CoV-2 inhibited inhibitory effect Interference nucleocapsid Nucleocapsid proteins plaque Plaque titration polysaccharide polysaccharides Propagation Quantitative Replication RNA copies RNA copy RT-PCR analysis Sargassum SARS-CoV-2 SARS-CoV-2 propagation SARS-CoV-2 replication SARS-CoV-2 spikes seaweed seaweed. Treatment Vero E6 VERO E6 cells viral entry viral infection viscera western blot [DOI] 10.3390/md20050296 PMC 바로가기 [Article Type] Article
Antibody responses to COVID-19 vaccines in older adultsArticle Published on 2022-04-012023-07-07 Journal: Journal of medical virology [Category] COVID19(2023년), [키워드] antibody Chemiluminescent microparticle immunoassay COVID-19 vaccine immunofluorescence assay older adults. [DOI] 10.1002/jmv.27531 PMC 바로가기
Development of an immunofluorescence assay for detection of SARS-CoV-2SARS-CoV-2 검출을 위한 면역형광 분석법 개발Article Published on 2022-04-012022-09-11 Journal: Archives of Virology [Category] COVID19(2023년), SARS, 치료기술, [키워드] 24 hour 24 hours approach assist can not carried Cell culture concentrations COVID-19 pandemic CPE CPE assay CPE assays Culture cultures Cytopathic effect defined demonstrated detect detectable determine development diagnostic test different time points dose etiologic agent identity IFA immunofluorescence assay immunofluorescence assay (IFA Increasing infected with SARS-CoV-2 Infection infectious virus infectious viruses infective Infective dose Initially nasopharyngeal occur Oropharyngeal pathogen Patient PCR assay positive Postinfection reduced reverse transcription SARS-CoV-2 sensitivity specificity Transmission Vero E6 Vero E6 culture Vero E6 cultures Viral RNA virus transmissibility while [DOI] 10.1007/s00705-022-05392-z PMC 바로가기 [Article Type] Article
Measurement of antibody levels in patients with COVID-19 over time by immunofluorescence assay: a longitudinal observational study면역형광 분석법을 통한 코로나19 환자의 시간 경과에 따른 항체 수치 측정: 종단적 관찰 연구Observational Study Published on 2022-01-012022-09-11 Journal: The Journal of international medical research [Category] SARS, 임상, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 addition age age and sex analyzed anti-SARS-CoV-2 antibody antibody antibody screening Antibody titer Antibody titers Chain Reaction coronavirus coronavirus disease Coronavirus disease 2019 Coronavirus-2 COVID-19 Critical examined fluorescent fluorescent antibody fluorescent antibody technique IgG IgG level Immunity immunofluorescence immunofluorescence assay Infection investigated lowest measurement occurred pandemic pathogenicity Patient patients with COVID-19 polymerase chain polymerase chain reaction positive positively correlated Post-infection Quantitative Reinfection respiratory reverse transcription reverse transcription polymerase chain reaction RT-qPCR SARS-CoV-2 serology severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus-2 severe acute respiratory syndrome coronavirus-2. Sex Standard deviation tested Variation were measured women [DOI] 10.1177/03000605211069279 PMC 바로가기 [Article Type] Observational Study